Bacterial DNA extraction

In order to examine a sample for foodborne pathogenic bacteria by molecular biological detection methods such as real-time PCR, bacterial DNA extraction has to be carried out first. The first step is always an enrichment of the food sample in liquid medium for about 1-2 days, followed by the isolation of bacterial DNA. Extraction of nucleic acids can be achieved very rapidly and with few handling steps with BIOTECON Diagnostics’ foodproof® ShortPrep Kits. These kits allow quick and easy sample preparation from numerous food matrices.

For bacterial DNA extraction from more critical sample matrices that contain PCR inhibitors, like e. g. spices, the foodproof® Sample Preparation Kits have been developed which generate highly purified DNA due to their filter tube principle. Several washing steps help eliminate cellular fragments, proteins and potentially inhibiting compounds that might otherwise cause false-negative PCR results.

BIOTECON Diagnostics does not only offer products for bacterial DNA extraction but also kits for yeast DNA isolation as well as for the extraction and purification of plant DNA for GMO analysis.

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Food testing  |  Beer analysis  |  GMO testing  |  Salmonella detection  |  Cronobacter detection
Listeria detection  |  Pathogen testing  |  Food PCR  |  Bacterial DNA extraction  |  PCR automation  |  EHEC detection