Salmonella detectionWith regard to the clinical and economical impact of food poisonings caused by Salmonella, sensitive and reliable Salmonella detection is one of the biggest concerns in the food industry. Salmonella are usually transmitted to humans by eating contaminated food of animal origin, such as beef, poultry, milk or eggs as well as any other food, including vegetables.
The genus Salmonella is part of the Enterobacteriaceae family and comprises the two species S. enterica and S. bongori. Salmonella enterica with its six subspecies is of primary clinical relevance for humans. The species is subdivided into more than 2,400 serovars defined by somatic and flagellar antigens, and most, if not all, of these serovars are considered pathogenic to animals and humans. However, Salmonella bongori has also been linked to cases of illness.
This diversity and pathogenicity of the genus gives rise to the demand for highly comprehensive and sensitive rapid methods for Salmonella detection. Since conventional microbiological methods for the detection and identification of Salmonella are very time-consuming, Salmonella detection by polymerase chain reaction (PCR) has been introduced to the food industry as a highly sensitive and specific rapid detection method. The foodproof® Salmonella Detection Kit is intended for the detection of Salmonella DNA isolated from enrichment cultures. In combination with the foodproof® ShortPrep I Kit for rapid sample preparation, this rapid microbiology method has been approved by the AOAC-RI and NordVal for a variety of foods.
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